Ikhithi yoVavanyo oluKhawulezayo lweSyphilis (Lateral Chromatography)

Iinkcukacha zeMveliso:

Usetyenziso olucetywayo:

I-Syphilis Rapid Test Kit (i-Lateral Chromatography) luvavanyo olukhawulezayo lwe-chromatographic immunoassay yokufumanisa umgangatho we-TP antibodies kwigazi lonke, i-serum okanye i-plasma ukunceda ukuxilongwa kwegcushuwa.

Imigaqo yoVavanyo:

I-Syphilis Rapid Test Kit isekwe kuvavanyo lwe-immunochromatographic ukufumana izilwa-buhlungu ze-TP kwigazi elipheleleyo, kwiserum okanye kwiplasma.Ngexesha lovavanyo, ii-antibodies ze-TP zidibana kunye ne-antigens ye-TP ebhalwe kumasuntswana anemibala engqukuva ukwenza i-immune complex.Ngenxa yesenzo se-capillary, i-immune complex iyahamba kwi-membrane.Ukuba isampuli iqulethe i-antibodies ye-TP, iya kubanjwa kwindawo yokuvavanya kwangaphambili kwaye yenze umgca wovavanyo olubonakalayo.Ukuze usebenze njengolawulo lwenkqubo, umgca wokulawula umbala uya kubonakala ukuba uvavanyo lwenziwe ngokufanelekileyo

Imixholo ephambili:

Ngomtya:

YeCassette:

Operation Flow

• Inyathelo 1: Ukulungiselela iSampuli

I-Syphilis Rapid Test Kit (iLateral Chromatography) inokwenziwa kusetyenziswa igazi elipheleleyo, i-serum okanye iplasma.

1. Ukwahlula i-serum okanye i-plasma egazini ngokukhawuleza kunokwenzeka ukuphepha i-hemolysis.Sebenzisa kuphela iisampulu ezicacileyo ezingenayo i-hemolyzed.

2. Uvavanyo kufuneka lwenziwe ngokukhawuleza emva kokuba isampuli ziqokelelwe.Ukuba uvavanyo alukwazi ukugqitywa ngokukhawuleza, i-serum kunye nesampuli ye-plasma kufuneka igcinwe kwi-2-8 ° C ukuya kwiintsuku ze-3, ukugcinwa kwexesha elide, imizekelo kufuneka igcinwe kwi -20 ℃.Igazi elipheleleyo eliqokelelwe nge-venipuncture kufuneka ligcinwe kwi-2-8 ° C ukuba uvavanyo luza kwenziwa kwiintsuku ezi-2 zokuqokelela.Musa ukukhenkcisa iisampuli zegazi elipheleleyo.Igazi elipheleleyo eliqokelelwe ngomnwe kufuneka lihlolwe ngoko nangoko.

3. Iisampulu kufuneka zibuyiselwe kwiqondo lobushushu begumbi phambi kovavanyo.Iisampulu ezikhenkcezisiweyo kufuneka zinyibilikiswe ngokupheleleyo kwaye zixutywe kakuhle phambi kovavanyo, kuthintelwe ukukhenkceza okuphindaphindiweyo kunye nokunyibilika.

4. Ukuba iisampulu ziza kuthunyelwa, kufuneka zipakishwe ngokuhambelana nemimiselo yendawo ejongene nokuthuthwa kwee-etiologic agents.

• Inyathelo lesi-2: Uvavanyo

Vumela umcu wovavanyo/ikhasethi, isampuli, idiluent yesampulu ifikelele kwigumbi

ubushushu (15-30 ° C) phambi kovavanyo.

1. Susa i-test strip/cassette kwisingxobo esivaliweyo kwaye uyisebenzise kwimizuzu engama-30.

2. Beka umtya wovavanyo/ikhasethi kwindawo ecocekileyo nekumgangatho.

2.1 NgeSerum okanye iMifanekiso yePlasma:

Bamba i-dropper ngokuthe nkqo, zoba isampuli ukuya kumgca wokuZalisa ongezantsi (malunga ne-40uL), kwaye udlulisele umboniso kumfanekiso wequla (S) lomtya wovavanyo/ikhasethi, emva koko wongeze ithontsi eli-1 lesampulu yediluent (malunga ne-40uL) kwaye uqale. isibali-xesha.Kuphephe ukubambisa amaqamza omoya kwiqula elingumzekelo (S).Jonga umfanekiso ongezantsi.

2.2 Ngegazi Elipheleleyo (iVenipuncture/iFingerstick) iisampulu:

Bamba idropha ngokuthe nkqo, zoba umboniso kumgca wokuZalisa ongaphezulu (malunga ne-80uL), kwaye udlulisele igazi elipheleleyo kwisampulu yequla (S) lovavanyo lomcu/ikhasethi, emva koko wongeze ithontsi eli-1 lesampulu ediluent (malunga ne-40uL) kwaye uqalise isibali-xesha.Kuphephe ukubambisa amaqamza omoya kwiqula elingumzekelo (S).Jonga umfanekiso ongezantsi.

• Inyathelo lesi-3: Ukufunda

3. Funda umphumo emva kwe-10-20 imizuzu.Isiphumo asisebenzi emva kwemizuzu engama-20.

Ukutolika iziphumo

1. Isiphumo Esilungileyo

Ukuba zombini umgangatho wolawulo lwe-C kunye nomgca we-T wokufumanisa ubonakala, kubonisa ukuba i-specimen iqulethe inani elibonakalayo le-antibodies ze-TP, kwaye umphumo ulungile kwi-syphilis.

2. Isiphumo esibi

Ukuba kuphela umgca we-C wokulawula umgangatho ubonakala kwaye umgca we-T wokufumanisa awubonisi umbala, ubonisa ukuba i-antibodies ye-TP ayibonakali kwi-specimen.kwaye isiphumo sibi kwigcushuwa.

3. Isiphumo esingasebenziyo

Akukho bhanti yembala ebonakalayo ibonakala kumgca wokulawula emva kokwenza uvavanyo, umphumo wokuvavanya awusebenzi.Phinda uvavanye isampuli.

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