Yeast Cell Protein Expression
Inkqubo yokubonakalisa igwele yindlela esetyenziswa ngokubanzi yokubonakalisa iprotein ye-eukaryotic, ngenxa yokulula kwayo ekulimeni, ukufikeleleka, kunye nokusebenza ngokulula.Phakathi kweentlobo ezahlukeneyo zegwele, i-Pichia pastoris yeyona nto idumileyo yokusingatha intetho, njengoko iququzelela zombini i-intracellular kunye ne-extracellular protein expression.Le nkqubo ikwavumela ukuguqulwa kwangemva kokuguqulelwa, okufana nephosphorylation kunye ne-glycosylation, okukhokelela kwinkqubo yokubonakalisa i-eukaryotic ekhethekileyo kunye neenzuzo ezininzi.
| Izinto zeNkonzo | Ixesha lokukhokela (BD) |
| Ukulungiswa kweCodon, i-gene synthesis kunye ne-subcloning | 5-10 |
| Ukuhlolwa kwe-clone okulungileyo | 10-15 |
| Inkcazo yesikali esincinci | |
| Isikali esikhulu (200ML) ukubonakaliswa kunye nokuhlanjululwa, ukuhanjiswa kubandakanya iprotheni ecocekileyo kunye nengxelo yovavanyo |
Ukuba i-gene yenziwe kwi-Bioantibody, i-plasmid eyakhiweyo iya kubandakanywa kwizinto ezinokuhanjiswa.