Inkonzo ye-Monoclonal Antibody
I-antibody ye-Monoclonal, eveliswa yi-B cell clone enye, ine-homogeneity ephezulu ejolise kwi-epitope ethile ye-antigen.I-antibody ye-Monoclonal ineenzuzo ezininzi, kubandakanya ukucoceka okuphezulu, ubuntununtunu, kunye neenkcukacha.Ii-Hybridomas zidalwe ngokudityaniswa kwee-lymphocyte ze-B ezivelisa izilwa-buhlungu ezithile, ezineeseli ze-myeloma ezihlala ixesha elide.I-Bioantibody isebenzisa itekhnoloji yodidi oluphezulu olusebenza ngamaxesha angama-20 kuneendlela eziqhelekileyo zokudityaniswa.Ukongeza, isebenzisa itekhnoloji yokuhlola iprotein ye-microarray ukuchonga ii-antibodies ze-monoclonal ezibonisa ukuchaneka okuphezulu, ukuhambelana, kunye nokusebenza ngempumelelo ngokuchasene neepitopes ezithile.
| Izinto zeNkonzo | Imixholo yoMfuniselo | Ixesha lokukhokela (Iveki) |
| Ukulungiswa kwe-Antigen | 1. UmThengi ubonelela nge-antigen2. I-Bioantibody ilungisa i-antigen | / |
| Ugonyo lwempuku | Ukugonywa kwe-BALB / c mouse, ukuqokelela i-serum kunye nohlalutyo lwe-ELISA | 4 |
| Ukudityaniswa kweeseli kunye nokuhlolwa | Ukudibanisa i-splenocytes ye-mouse kunye neeseli ze-myeloma, ukuhlolwa kwe-HAT | 2 |
| Ukusekwa kwe-cell line ezinzileyo | Ukuhlanganiswa kwee-clones eziqinisekisiweyo | 3 |
| Ukuchongwa kwe-isotype ye-antibody | Ukuchongwa kwee-subtypes ze-cell line | 1 |
| I-incubation yesikali esincinci | I-incubation engenaserum | 2 |
| I-incubation enkulu kunye nokucoca | I-200mL i-serum-free incubation kunye nokucoca | 1 |
