Recombinant Rotavirus A Intermediate Capsid Protein VP6, N-Ithegi yakhe

Thumela i-imeyile kuthi

Iinkcukacha zeMveliso

Umthombo	Umntu
Isivakalisi sokuvakalisa	Rotavirus A
Ithegi	N-Ithegi yakhe
Isicelo	Ilungele ukusetyenziswa kwi-immunoassays. Ilabhoratri nganye kufuneka igqibe ngesona sihloko sisebenza ngokufanelekileyo ukuze isetyenziswe kwimisebenzi yayo.
Iinkcukacha zajikelele	Recombinant rotavirus A intermediate capsid protein VP6 uveliswa E.coli expression system kunye nethagethi yejini encoding Met1-Lys397 ibonakaliswa nge His-tag kwi-N-terminus.

Ubunyulu	>90% njengoko kumiselwe yi-SDS-PAGE.
IMisa yeMolekyuli	I-recombinant rotavirus A Intermediate Capsid Protein VP6 ebandakanya i-412 amino acids kwaye inobunzima bemolekyuli obaliweyo malunga ne-46.6 kDa.
Isithinteli seMveliso	10 mM PB, 6% sucrose, pH 8.0.
Ugcino	Yigcine phantsi kweemeko ezingakhuselekanga ku -20℃ ukuya -80℃ xa uyifumene. Ncoma ukuba i-aliquot iprotheni ibe yimiyinge emincinci yokugcina eyona ilungileyo.