I-Recombinant Monkeypox Virus B6R Protein, C-Ithegi yakhe

Thumela i-imeyile kuthi

Iinkcukacha zeMveliso

Umthombo	Intsholongwane yeMonkeypox(strain Zaire-96-I-16)
Isivakalisi sokuvakalisa	HEK 293 Iiseli
Ithegi	C-Ithegi yakhe
Isicelo	Ilungele ukusetyenziswa kwi-immunoassays. Ilabhoratri nganye kufuneka igqibe ngesona sihloko sisebenza ngokufanelekileyo ukuze isetyenziswe kwimisebenzi yayo.
Iinkcukacha zajikelele	I-recombinant monkeypox virus ye-B6R iprotheni iveliswa yi-Mammalianexpression system kunye ne-target gene encoding Tyr18-Thr277 ibonakaliswe nge-tag yakhe kwi-C-terminus.

Ubunyulu	>95% njengoko kumiselwe yiSDS-PAGE.
IMisa yeMolekyuli	Iprotheni ye-Recombinant Monkeypox Virus B6R equkethe i-275 amino acids kwaye inobunzima be-molecular mass 30.5 kDa.Iprotheni ifuduka njenge-40.0 kDa phantsi kokunciphisa i-SDS-PAGE ngenxa ye-glycosylation.
Isithinteli seMveliso	10 mM PB, 300 mM NaCl, pH 8.0.
Ugcino	Yigcine phantsi kweemeko ezingakhuselekanga ku -20℃ ukuya -80℃ xa uyifumene. Ncoma ukuba i-aliquot iprotheni ibe yimiyinge emincinci yokugcina eyona ilungileyo.