I-Recombinant Monkeypox Virus A35R Protein, C-Ithegi yakhe

Thumela i-imeyile kuthi

Iinkcukacha zeMveliso

Umthombo	Intsholongwane yeMonkeypox(strain Zaire-96-I-16)
Isivakalisi sokuvakalisa	HEK 293 Iiseli
Ithegi	C-Ithegi yakhe
Isicelo	Ilungele ukusetyenziswa kwi-immunoassays. Ilabhoratri nganye kufuneka igqibe ngesona sihloko sisebenza ngokufanelekileyo ukuze isetyenziswe kwimisebenzi yayo.
Iinkcukacha zajikelele	I-recombinant monkeypox virus A35R protein iveliswa yi-Mammalian expression system kunye ijini ekujoliswe kuyo i-encoding Arg58-Thr181 ichazwa nge-His-tag kwi-C-terminus.

Ubunyulu	>95% njengoko kumiselwe yiSDS-PAGE.
IMisa yeMolekyuli	I-recombinant monkeypox virus i-A35R iprotheni equkethe i-139 amino acids kwaye inobunzima obubaliweyo be-molecular 15.3 kDa.Iprotheni ifuduka njenge-15-26 kDa phantsi kokunciphisa i-SDS-PAGE ngenxa ye-glycosylation.
Isithinteli seMveliso	20 mM Tris, 10 mM NaCl, pH 8.0.
Ugcino	Yigcine phantsi kweemeko ezingakhuselekanga ku -20℃ ukuya -80℃ xa uyifumene. Ncoma ukuba i-aliquot iprotheni ibe yimiyinge emincinci yokugcina eyona ilungileyo.