I-Recombinant Monkeypox Virus A29L Protein, C-Ithegi yakhe

Thumela i-imeyile kuthi

Iinkcukacha zeMveliso

Umthombo	Intsholongwane yeMonkeypox(strain Zaire-96-I-16)
Isivakalisi sokuvakalisa	E.coli
Ithegi	C-Ithegi yakhe
Isicelo	Ilungele ukusetyenziswa kwi-immunoassays. Ilabhoratri nganye kufuneka igqibe ngesona sihloko sisebenza ngokufanelekileyo ukuze isetyenziswe kwimisebenzi yayo.
Iinkcukacha zajikelele	Recombinant monkeypox virus A29L protein iveliswa yi E.coli expression system kunye ne ijene ekujoliswe kuyo encoding Met1-Glu110 ichazwa nge His-tag kwi C-terminus.

Ubunyulu	>95% njengoko kumiselwe yiSDS-PAGE.
IMisa yeMolekyuli	I-recombinant monkeypox virus i-A29L yeprotheni equkethe i-125 amino acids kwaye inobunzima obubaliweyo be-molecular 14.2 kDa.
Isithinteli seMveliso	20 mM Tris, 300 mM NaCl, pH 9.0.
Ugcino	Yigcine phantsi kweemeko ezingakhuselekanga ku -20℃ ukuya -80℃ xa uyifumene. Ncoma ukuba i-aliquot iprotheni ibe yimiyinge emincinci yokugcina eyona ilungileyo.