I-Recombinant Helicobacter pylori UreB protein, N-Ithegi yakhe

Thumela i-imeyile kuthi

Iinkcukacha zeMveliso

Umthombo	Umntu
Isivakalisi sokuvakalisa	E.coli
Ithegi	N-Ithegi yakhe
Isicelo	Ilungele ukusetyenziswa kwi-immunoassays. Ilabhoratri nganye kufuneka igqibe ngesona sihloko sisebenza ngokufanelekileyo ukuze isetyenziswe kwimisebenzi yayo.
Iinkcukacha zajikelele	I-recombinant helicobacter pylori UreB protein iveliswa yi-E.coli expression system kunye ne-target gene encoding Met1-Phe569 ibonakaliswa nge-His-tag kwi-N-terminus.

Ubunyulu	>95% njengoko kumiselwe yiSDS-PAGE.
IMisa yeMolekyuli	I-recombinant helicobacter pylori UreB protein iqukethe i-583 amino acids kwaye inobunzima bemolekyuli obalwayo malunga ne-63.3 kDa.
Isithinteli seMveliso	20 mM Tris, 10 mM NaCl, 3% Glycerol, pH 9.0.
Ugcino	Yigcine phantsi kweemeko ezingakhuselekanga ku -20℃ ukuya -80℃ xa uyifumene. Ncoma ukuba i-aliquot iprotheni ibe yimiyinge emincinci yokugcina eyona ilungileyo.