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I-Recombinant Helicobacter pylori CagA protein, N-Ithegi yakhe

Inkcazelo emfutshane:


Iinkcukacha zeMveliso

Iithegi zeMveliso

Iinkcukacha zajikelele

Umthombo Umntu
Isivakalisi sokuvakalisa E.coli
Ithegi N-Ithegi yakhe
Isicelo Ilungele ukusetyenziswa kwi-immunoassays. Ilabhoratri nganye kufuneka imisele inqaku elisebenza ngokufanelekileyo ukuze lisetyenziswe kwizicelo zayo.
Iinkcukacha zajikelele I-Recombinant Helicobacter pylori CagA iveliswa yi-E.coli expression system kunye ne-targetgene encoding i-Met1-Phe294 ibonakaliswa nge-His-tag kwi-N-terminus.

Iipropati

Ubunyulu >90% njengoko kumiselwe yi-SDS-PAGE.

8

Imolekyuli IMisa

Iprotheni ye-helicobacter pylori CagA iqukethe i-308 amino acids kwaye inobunzima obubaliweyo be-molecular malunga ne-35.2 kDa.
Isithinteli seMveliso 10 mM PB, pH 8.0.
Ugcino Yigcine phantsi kweemeko ezingakhuselekanga ku -20℃ ukuya -80℃ xa uyifumene.

Ncoma ukuba i-aliquot iprotheni ibe yimiyinge emincinci yokugcina eyona ilungileyo.

Ulwazi lweodolo

Igama lemveliso Ikati.Hayi Ubungakanani
 

I-Recombinant Helicobacter pylori CagA protein, N-Ithegi yakhe

 

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