I-Recombinant Helicobacter pylori HSP60 protein, C-Ithegi yakhe

Thumela i-imeyile kuthi

Iinkcukacha zeMveliso

Umthombo	Helicobacter pylori
Isivakalisi sokuvakalisa	E.coli
Ithegi	C-Ithegi yakhe
Isicelo	Ilungele ukusetyenziswa kwi-immunoassays. Ilabhoratri nganye kufuneka igqibe ngesona sihloko sisebenza ngokufanelekileyo ukuze isetyenziswe kwimisebenzi yayo.
Iinkcukacha zajikelele	I-recombinant helicobacter pylori HSP60 iveliswa yi-E.coli expression system kunye nethagethi gene encoding Met1-Met546 ichazwa nge His-tag kwi C-terminus.

Ubunyulu	>95% njengoko kumiselwe yiSDS-PAGE.
IMisa yeMolekyuli	I-recombinant helicobacter pylori HSP60 protein iqukethe i-560 amino acids kwaye inobunzima bemolekyuli obaliweyo malunga ne-59.8 kDa.
Isithinteli seMveliso	10 mM PB, 300 mM NaCl, 5% Glycerol, pH 7.0.
Ugcino	Yigcine phantsi kweemeko ezingakhuselekanga ku -20℃ ukuya -80℃ xa uyifumene. Ncoma ukuba i-aliquot iprotheni ibe yimiyinge emincinci yokugcina eyona ilungileyo.