Ikhithi yoVavanyo lwe-Antibody yeLeishmania IgG/IgM (Uvavanyo lwe-Immunochromatographic)

Inkcazo emfutshane:

Isampuli	I-Serum/iPlasma/iGazi elipheleleyo	Ifomathi	Ikhasethi
Uvelwano	98.83%	Ukuchaneka	98.02%
Ubushushu beTrans. kunye neSto.	2-30℃ / 36-86℉	Ixesha lovavanyo	imizuzu emi-5 ukuya kweli-10
Inkcazo	Uvavanyo/Ikhithi e-1 Uvavanyo/Ikhithi e-5 Uvavanyo/Ikhithi e-25 Uvavanyo/Ikhithi

Thumela i-imeyile kuthi

Iinkcukacha zeMveliso

Iithegi zeMveliso

Iinkcukacha zemveliso

Ukusetyenziswa Okucetywayo
Le mveliso ifanelekile ekuhlolweni kweklinikhi okusemgangathweni kweesampuli ze-serum/plasma/zegazi elipheleleyo ukuze kufunyanwe ii-antibodies ezichasene neLeishmania. Luvavanyo olulula, olukhawulezayo nolungasebenzisi izixhobo zokuchonga i-kala-azar ebangelwa yiLeishmania.

Umgaqo woVavanyo
Le mveliso yi-lateral flow chromatographic immunoassay. Ikhasethi yovavanyo iqulathe oku kulandelayo: 1) i-conjugate pad enemibala e-burgundy equlethe i-recombinant rK39 antigen edityaniswe negolide ye-colloid (ii-Leishmania conjugates) kunye nee-conjugates zegolide ye-rabbit IgG; 2) i-nitrocellulose membrane strip equlathe ii-test bands ezimbini (ii-M kunye nee-G bands) kunye ne-control band (i-C band).

Isiqulatho esiPhambili

Izinto ezibonelelweyo zidweliswe kwitheyibhile.

Izixhobo / ezibonelelweyo	Ubungakanani (Uvavanyo/Ikhithi e-1)	Ubungakanani (5Uvavanyo/Ikhithi)	Ubungakanani (25Uvavanyo/Ikhithi)
Ikhithi yovavanyo	Uvavanyo olu-1	Uvavanyo olu-5	Uvavanyo oluyi-25
I-Buffer	Ibhotile e-1	Iibhotile ezi-5	Iibhotile ezingama-25/2
Idropper	Isiqwenga esi-1	5 ii-pcs	25 ii-pcs
Ingxowa yoThutho yeMizekelo	Isiqwenga esi-1	5 ii-pcs	25 ii-pcs
I-Lancet elahlwayo	Isiqwenga esi-1	5 ii-pcs	25 ii-pcs
Imiyalelo Yokusetyenziswa	Isiqwenga esi-1	Isiqwenga esi-1	Isiqwenga esi-1
Isatifikethi sokuThotyelwa kweMithetho	Isiqwenga esi-1	Isiqwenga esi-1	Isiqwenga esi-1

Ukuhamba Komsebenzi

Inyathelo 1: Ukuthatha isampuli

Qokelela iSerum/Plasma/Igazi elipheleleyo lomntu ngokufanelekileyo.

Inyathelo lesi-2: Uvavanyo

Nceda ufunde imiyalelo ngononophelo ngaphambi kokuvavanya. Ngaphambi kokuvavanya, vumela ikhithi yovavanyo, isisombululo sesampuli kunye nesampuli ukuba zilingane nobushushu (15-30℃ okanye 59-86 degrees Fahrenheit).
1. Susa ityhubhu yokukhupha kwikhithi kunye nebhokisi yovavanyo kwingxowa yefilimu ngokukrazula i-notch. Zibeke kwindawo ethe tye.
2. Vula ibhegi yefoyile ye-aluminium yekhadi lokuhlola. Susa ikhadi lovavanyo uze ulibeke ngokuthe tye etafileni.
3.Sebenzisa ipayipi elahlwayo, dlulisela ithontsi elinye (malunga ne-20μL) legazi elisezintendeni zomnwe/okanye i-4μL serum/okanye i-4μL plasma/ okanye i-4μL igazi elipheleleyo kwiqula lesampulu elikwikhasethi yovavanyo.
4. Vula ityhubhu ye-buffer. Faka amathontsi ama-3 (malunga ne-80 μL) e-assay diluent kwi-assay diluent emile kakuhle ingqukuva. Qala ukubala.

Inyathelo lesi-3: Ukufunda

Funda iziphumo emva kwemizuzu emi-5-10. Iziphumo emva kwemizuzu eli-10 azisebenzi.

Ingcaciso yeziphumo

Iziphumo ezingalunganga
Ukuba kuphela umgca wolawulo lomgangatho u-C uvela kwaye imigca yokuchonga u-G no-M ayibonakali

Isiphumo Esihle
1. Zombini umgca wokulawula umgangatho C kunye nomgca wokufumanisa uM ziyavela= i-antibody yeLeishmania IgM ifunyenwe, kwaye iziphumo zilungile kwi-antibody ye-IgM.
2. Zombini umgca wokulawula umgangatho C kunye nomgca wokufumanisa uG ziyavela= i-antibody yeLeishmania IgG ifunyenwe kwaye iziphumo zilungile kwi-antibody ye-IgG.
3. Zombini umgca wokulawula umgangatho C kunye nemigca yokufumanisa uG kunye no-M ziyavela=ii-antibodies zeLeishmania IgG kunye ne-IgM. ziyafunyanwa, kwaye isiphumo siba sihle kwii-antibodies ze-IgG kunye ne-IgM.

Isiphumo Esingasebenziyo
Umgca wolawulo lomgangatho C awunakubonwa, iziphumo aziyi kuba zezokusebenza nokuba umgca wovavanyo uvela na, kwaye uvavanyo kufuneka luphindwe.

Ulwazi lwe-oda

Igama lemveliso	Ikati. Hayi	Ubungakanani	Isampuli	Beka ubomi kwishelufa	Ubushushu beTrans. kunye neSto.
Ikhithi yoVavanyo lwe-Antibody yeLeishmania IgG/IgM (Uvavanyo lwe-Immunochromatographic)	B020C-01	Uvavanyo olu-1/ikhithi	I-Serum/iPlasma/iGazi elipheleleyo	Iinyanga ezili-18	2-30℃ / 36-86℉
	B020C-05	Uvavanyo olu-5/ikhithi
	B020C-25	Uvavanyo lwe-25/ikhithi