Ikhithi yoVavanyo oluKhawulezayo lweNtsholongwane yeMonkeypox Virus (iLateral Chromatography)
Iinkcukacha zemveliso
Ukusetyenziswa Okucetywayo:
I-Monkeypox Virus Antigen Rapid Test Kit isetyenziselwa ukufumanisa i-Monkeypox antigen esemgangathweni kwiisampuli ze-exudate okanye ze-scab zabantu. Yenzelwe ukusetyenziswa ekuxilongweni kwe-in vitro kuphela.
Imigaqo yoVavanyo:
Xa isampuli icutshungulwa kwaye yongezwa kwiqula lesampuli, ii-antigen zentsholongwane ye-monkeypox kwisampuli ziyasebenzisana ne-conjugate ye-antibody yentsholongwane ye-monkeypox eyenza i-antigen-antibody color particle complexes. Ezi complexes zihamba kwi-membrane ye-nitrocellulose ngesenzo se-capillary ukuya kumgca wovavanyo, apho zibanjwa khona yi-mouse monoclonal anti-monkeypox virus antibodies. Umgca wovavanyo onemibala ubonakala kwifestile yesiphumo ukuba ii-antigen zentsholongwane ye-monkeypox zikhona kwisampuli kwaye ubunzulu buxhomekeke kubungakanani be-antigen yentsholongwane ye-monkeypox. Xa ii-antigen zentsholongwane ye-monkeypox kwisampuli zingekho okanye zingaphantsi komda wokufunyanwa, akukho band enemibala ebonakalayo kumgca wovavanyo wesixhobo. Oku kubonisa isiphumo esibi. Umgca wovavanyo okanye umgca wolawulo awubonakali kwifestile yesiphumo ngaphambi kokusebenzisa isampuli. Umgca wolawulo obonakalayo uyafuneka ukubonisa ukuba isiphumo siyasebenza.
Isiqulatho esiPhambili
Izinto ezibonelelweyo zidweliswe kwitheyibhile.
| I-REF yeCandelo | B031C-01-01 | B031C-01-25 | B031C-01-25 |
| Ikhasethi yovavanyo | Uvavanyo olu-1 | Uvavanyo lwe-5 | Uvavanyo lwe-25 |
| Isisombululo sokukhupha isampulu | Ibhotile e-1 | Ibhotile ezi-5 | Ibhotile engama-25 |
| Iswabhu yokulahla | Isiqwenga esi-1 | Iziqwenga ezi-5 | Iziqwenga ezingama-25 |
| Imiyalelo yokusebenzisa | Isiqwenga esi-1 | Isiqwenga esi-1 | Isiqwenga esi-1 |
| Isatifikethi sokuvumelana | Isiqwenga esi-1 | Isiqwenga esi-1 | Isiqwenga esi-1 |
Ukuhamba Komsebenzi
Funda imiyalelo kakuhle ngaphambi kokuvavanya uze uzise ikhasethi kunye nesampuli kubushushu begumbi. Xa sele ukulungele ukuvavanya, vula isikhwama esikwinotshi uze ususe isixhobo. Beka isixhobo sokuvavanya kwindawo ecocekileyo, ethe tyaba.
I-Monkeypox Virus Antigen Rapid Test Kit inokwenziwa kwisampuli ze-lesion exudate okanye ze-scab.
NgokubaIisampulu ze-lesion exudate okanye i-scab:
1. Sula iisampulu ze-exudate yesilonda okanye iisampulu ze-scab nge-swab.
2. Faka iswabhu kwityhubhu yesisombululo sokukhupha isampuli uze uyifake phezulu nasezantsi kulwelo ubuncinane imizuzwana eli-15, uze ubambe iswabhu ezantsi kwityhubhu uze uyijikelezise izihlandlo ezi-5.
3. Susa i-swab ngelixa ucinezela amacala etyhubhu ukuze ukhuphe ulwelo kwi-swab. Cinezela isiciko somlomo ngokuqinileyo kwityhubhu yesisombululo equlethe isampuli ecutshungulweyo. Xuba kakuhle ngokujikelezisa okanye ngokushukumisa ezantsi kwetyhubhu.
4. Xuba isampuli ngokuyijika kancinci ityhubhu ijonge ezantsi, ucinezele ityhubhu ukuze wongeze amathontsi ama-3 (malunga ne-100 μL) kwiqula lesampuli yekhasethi yovavanyo, uze uqale ukubala.
5. Funda iziphumo ngokujonga emva kwemizuzu eli-15-20. Iziphumo azisebenzi emva kwemizuzu engama-20.
Ukutolika iziphumo
Ulwazi lwe-oda
| Igama lemveliso | Ikati. Hayi | Ubungakanani | Isampuli | Beka ubomi kwishelufa | Ubushushu beTrans. kunye neSto. |
| Ikhithi yoVavanyo oluKhawulezayo lweNtsholongwane yeMonkeypox Virus | B031C-01 | Uvavanyo/ikhithi e-1 | iisampulu ze-lesion exudate okanye i-scab | Iinyanga ezingama-24 | 2-30℃ |
| B031C-05 | Uvavanyo/ikhithi ezi-5 | ||||
| B031C-25 | Uvavanyo/ikhithi engama-25 |
