Ikhithi yoVavanyo oluKhawulezayo lweTroponin I yeCardiac (iChromatography esecaleni) Umfanekiso obonisiweyo

Ikhithi yoVavanyo oluKhawulezayo lweTroponin I yentliziyo (iChromatography esecaleni)

Inkcazo emfutshane:

Isampuli	I-Serum/iPlasma/iGazi elipheleleyo	Ifomathi	Ikhasethi
Uvelwano	99.60%	Ukuchaneka	98.08%
Ubushushu beTrans. kunye neSto.	2-30℃ / 36-86℉	Ixesha lovavanyo	imizuzu eli-10-30
Inkcazo	Uvavanyo/Ikhithi e-1; Uvavanyo/Ikhithi engama-25

Thumela i-imeyile kuthi

Iinkcukacha zeMveliso

Iithegi zeMveliso

Iinkcukacha zemveliso

Ukusetyenziswa Okucetywayo:

I-Cardiac Troponin I Rapid Test Kit isebenzisa i-colloidal gold immunochromatography ukuze ibone i-cardiac Troponin I (cTnI) kwi-serum, kwi-plasma okanye kwisampulu yegazi liphela ngokomgangatho okanye ngokwesiqingatha ngekhadi eliqhelekileyo le-colorimetric. Olu vavanyo lusetyenziswa njengoncedo ekuxilongweni kokwenzakala kwentliziyo okufana ne-Acute Myocardial Infarction, i-Unstable Angina, i-Acute Myocarditis kunye ne-Acute Coronary Syndrome.

Imigaqo yoVavanyo:

I-Cardiac Troponin I Rapid Test Kit (i-Lateral Chromatography) yi-immunoassay esekwe kwi-membrane okanye ekumgangatho ophantsi, esetyenziselwa ukufumanisa i-cardiac Troponin I(cTnI) egazini elipheleleyo, kwi-serum okanye kwi-plasma. Kule nkqubo yovavanyo, i-capture reagent ayishukumi kwindawo yomgca wovavanyo wovavanyo. Emva kokuba isampuli yongezwe kwindawo yesampuli yekhasethi, isabela kunye namaqhekeza agqunywe yi-anti-cTnI antibody kuvavanyo. Lo mxube uhamba nge-chromatographically kubude bovavanyo kwaye usebenzisana ne-immobilized capture reagent. Ifomathi yovavanyo inokubhaqa i-cardiac Troponin I(cTnI) kwi-sampuli. Ukuba isampuli ine-cardiac Troponin I(cTnI), umgca onemibala uya kuvela kwindawo yomgca wovavanyo kwaye ubukhulu bombala bomgca wovavanyo buyanda ngokulingana noxinzelelo lwe-cTnI, nto leyo ebonisa iziphumo ezilungileyo. Ukuba isampuli ayinayo i-cardiac Troponin I(cTnI), umgca onemibala awuyi kuvela kule ndawo, nto leyo ebonisa iziphumo ezimbi. Ukuze isebenze njengolawulo lwenkqubo, umgca onemibala uya kuhlala uvela kummandla womgca wolawulo, nto leyo ebonisa ukuba kufakwe umthamo ofanelekileyo wesampuli kwaye kuye kwenzeka ukukrwela kwe-membrane.

Isiqulatho esiPhambili

Izinto ezibonelelweyo zidweliswe kwitheyibhile.

I-REF yeCandelo I-REF	B032C-01	B032C-25
Ikhasethi yovavanyo	Uvavanyo olu-1	Uvavanyo oluyi-25
Isampulu yokuxuba	Ibhotile e-1	Ibhotile e-1
Idropper	Isiqwenga esi-1	25 ii-pcs
Ikhadi lemibala eliqhelekileyo	Isiqwenga esi-1	Isiqwenga esi-1
Isatifikethi sokuThotyelwa kweMithetho	Isiqwenga esi-1	Isiqwenga esi-1

Ukuhamba Komsebenzi

Inyathelo 1: Ukulungiselela iSampulu

1. Ikhithi yovavanyo ingenziwa kusetyenziswa igazi elipheleleyo, i-serum okanye i-plasma. Cebisa ukuba ukhethe i-serum okanye i-plasma njengesampulu yovavanyo. Ukuba ukhetha igazi elipheleleyo njengesampulu yovavanyo, kufuneka isetyenziswe kunye ne-diluent yesampulu yegazi.

2. Vavanya isampuli ekwikhadi lovavanyo ngoko nangoko. Ukuba uvavanyo alunakugqitywa ngoko nangoko, isampuli yeserum kunye neplasma kufuneka igcinwe ukuya kuthi ga kwiintsuku ezisi-7 kwi-2~8℃ okanye igcinwe kwi--20℃ kangangeenyanga ezi-6 (isampuli yegazi elipheleleyo kufuneka igcinwe ukuya kuthi ga kwiintsuku ezi-3 kwi-2~8℃) ide ivavanywe.

3. Iisampulu kufuneka zibuyiselwe kubushushu begumbi ngaphambi kovavanyo. Iisampulu ezikhenkcezisiweyo kufuneka zinyibilikiswe ngokupheleleyo kwaye zixutywe kakuhle ngaphambi kovavanyo, kuthintelwe ukukhenkcezwa nokunyibilikiswa rhoqo.

4. Kuphephe ukufudumeza iisampuli, nto leyo enokubangela i-hemolysis kunye nokuguquka kweproteni. Kucetyiswa ukuba kuphephe ukusebenzisa isampuli efakwe i-hemolyzed kakhulu. Ukuba isampuli ibonakala ngathi ifakwe i-hemolyzed kakhulu, kufuneka kufunyanwe enye isampuli ize ivavanywe.

Inyathelo lesi-2: Uvavanyo

1. Nceda ufunde le ncwadi yemiyalelo ngononophelo ngaphambi kokuvavanya, buyisela isampulu, ikhadi lovavanyo kunye nesampuli yegazi elixutyiweyo kubushushu begumbi uze ufake inombolo kwikhadi. Cebisa ukuvula ingxowa yefoyile emva kokuba ibuyele kubushushu begumbi kwaye usebenzise ikhadi lovavanyo ngoko nangoko.

2. Beka ikhadi lovavanyo etafileni ecocekileyo, ibekwe ngokuthe tye.

Kwisampuli yeSerum okanye yePlasma:

Bamba idropha ngokuthe nkqo uze udlulisele amathontsi amathathu e-serum okanye i-plasma (malunga ne-80 L, iPipette ingasetyenziswa xa kungxamisekile) kwiqula lesampuli, uze uqalise isibali-xesha. Jonga umzekeliso ongezantsi.

Kwisampuli yeGazi Elipheleleyo:

Bamba idropha ngokuthe nkqo uze udlulisele amathontsi amathathu egazi elipheleleyo (malunga ne-80 L) kwisampuli equlathe amanzi, uze wongeze ithontsi eli-1 le-sample diluent (malunga ne-40 L), uze uqalise isibali-xesha. Jonga umzekeliso ongezantsi.

Inyathelo lesi-3: Ukufunda

Kwimizuzu eli-10 ukuya kwengama-30, fumana isiphumo esilinganiselweyo ngokwekhadi lemibala eliqhelekileyo ngamehlo.

Ukutolika iziphumo

Iyasebenza: Kuvela umgca obomvu omfusa kumgca wolawulo (C). Ngokuphathelele iziphumo ezichanekileyo, ungafumana i-semi-quantitative by eyes ngekhadi eliqhelekileyo le-colorimetric:

Ubungakanani boMbala vs Ugxininiso lweReferensi

Ubungakanani bombala	Ugxininiso lweReferensi (ng / ml)
-	＜0.5
+ -	0.5~1
+	1~5
+ +	5~15
+ + +	15~30
+ + + +	30~50
+ + + +	＞50

Ayisebenzi: Akukho migca ibomvu emfusa ibonakala kumgca wolawulo (C). Oku kuthetha ukuba ezinye iindlela zokusebenza kufuneka zingachanekanga okanye ikhadi lovavanyo sele lingasebenzi. Kule meko nceda ufunde incwadi yemiyalelo ngononophelo kwakhona, uze uzame kwakhona ngekhasethi entsha yovavanyo. Ukuba imeko efanayo yenzekile kwakhona, kufuneka uyeke ukusebenzisa le mveliso ngoko nangoko kwaye unxibelelane nomthengisi wakho.

Ulwazi lwe-oda

Igama lemveliso	Ikati. Hayi	Ubungakanani	Isampuli	Beka ubomi kwishelufa	Ubushushu beTrans. kunye neSto.
Ikhithi yoVavanyo oluKhawulezayo lweTroponin I yentliziyo (iChromatography esecaleni)	B032C-01	Uvavanyo/ikhithi e-1	I-S/P/WB	Iinyanga ezingama-24	2-30℃
	B032C-25	Uvavanyo lwe-25/ikhithi	I-S/P/WB	Iinyanga ezingama-24	2-30℃