I-Cardiac Troponin I Kit yoVavanyo oluKhawulezayo (Lateral Chromatography)
Iinkcukacha zemveliso
Usetyenziso olucetywayo:
I-Cardiac Troponin I-Rapid Test Kit isebenzisa i-immunochromatography yegolide ye-colloidal ukukhangela intliziyo ye-Troponin I (cTnI) kwi-serum, i-plasma okanye isampuli yegazi elipheleleyo ngokomgangatho okanye i-semi-quantitatively kunye nekhadi eliqhelekileyo lemibala.Olu vavanyo lusetyenziswa njengoncedo ekuxilongweni kokulimala kwe-myocardial efana ne-Acute Myocardial Infarction, i-Angina engazinzanga, i-Acute Myocarditis kunye ne-Acute Coronary Syndrome.
Imigaqo yoVavanyo:
ICardiac Troponin I Rapid Test Kit (Lateral Chromatography) isemgangathweni okanye i-semi-quantitative, i-membrane esekelwe kwi-immunoassay yokufumanisa i-cardiac Troponin I (cTnI) kwigazi elipheleleyo, i-serum okanye i-plasma.Kule nkqubo yovavanyo, i-reagent yokubamba i-immobilized kwindawo yovavanyo lovavanyo.Emva kokuba i-specimen yongezwe kwindawo ye-specimen yekhasethi, isabela kunye ne-anti-cTnI antibody coated particles kuvavanyo.Lo mxube ufuduka ngokwekhromatografi ngobude bovavanyo kwaye unxibelelane ne-reagent yokubamba engenakunyakaziswa.Ifomathi yovavanyo inokubona iTroponin I(cTnI) yentliziyo kwimizekelo.Ukuba i-specimen iqulethe i-cardiac Troponin I (cTnI), umgca onemibala uya kubonakala kwingingqi yomgca wokuvavanya kwaye ubukhulu bombala bomgca wokuvavanya buyanda ngokulingana ne-cTnI yoxinaniso, ebonisa umphumo omuhle.Ukuba umzekelo awuqukethe i-cardiac Troponin I (cTnI), umgca onemibala awuyi kuvela kulo mmandla, ebonisa umphumo ombi.Ukuze usebenze njengolawulo lwenkqubo, umgca onemibala uya kuhlala ubonakala kummandla wolawulo, obonisa ukuba umthamo ofanelekileyo we-specimen wongeziwe kwaye i-membrane wicking yenzeke.
Imixholo ephambili
Amacandelo anikiweyo adweliswe kwitheyibhile.
| Icandelo REF I-REF | B032C-01 | B032C-25 |
| Ikhasethi yovavanyo | 1 uvavanyo | Iimvavanyo ezingama-25 |
| Isampulu yokuxutywa | 1 ibhotile | 1 ibhotile |
| Idropha | Iqhekeza eli-1 | 25 iipcs |
| Ikhadi eliqhelekileyo lemibala | Iqhekeza eli-1 | Iqhekeza eli-1 |
| Isatifikethi sokuThobela | Iqhekeza eli-1 | Iqhekeza eli-1 |
Operation Flow
Inyathelo 1: Ukulungiselela iSampuli
1. Ikiti yokuvavanya inokwenziwa kusetyenziswa igazi elipheleleyo, i-serum okanye i-plasma.Cebisa ukukhetha i-serum okanye iplasma njengesampulu yovavanyo.Ukuba ukhetha igazi elipheleleyo njengesampulu yovavanyo, kufuneka lisetyenziswe kunye nesampulu yegazi lokuthambisa.
2. Vavanya isampuli kwikhadi lovavanyo ngoko nangoko.Ukuba uvavanyo alukwazi ukugqitywa ngokukhawuleza, i-serum kunye nesampuli ye-plasma kufuneka igcinwe ukuya kwiintsuku ze-7 kwi-2 ~ 8 ℃ okanye igcinwe kwi-20 ℃ kwiinyanga ze-6 (isampuli yegazi lonke kufuneka igcinwe ukuya kwiintsuku ze-3 kwi-2 ~ 8 ℃ ) de ivavanywe.
3. Iisampulu kufuneka zibuyiselwe kwiqondo lobushushu begumbi phambi kovavanyo.Iisampulu ezikhenkcezisiweyo kufuneka zinyibilikiswe ngokupheleleyo kwaye zixutywe kakuhle phambi kovavanyo, kuthintelwe ukukhenkceza okuphindaphindiweyo kunye nokunyibilika.
4. Gwema ukufudumeza iisampuli, ezinokubangela i-hemolysis kunye ne-protein denaturation.Kucetyiswa ukuba uphephe ukusebenzisa isampulu ye-hemolyzed kakhulu.Ukuba isampulu ibonakala ngathi ine-hemolyzed kakhulu, enye isampuli kufuneka ifunyanwe kwaye ivavanywe.
Inyathelo lesi-2: Uvavanyo
1. Kucelwa ufunde le ncwadana ngononophelo phambi kovavanyo, buyisela isampuli, ikhadi lovavanyo kunye nesampuli yegazi diluent kwiqondo lobushushu begumbi kunye nenombolo yekhadi.Cebisa ukuvula isikhwama se-foil emva kokuba sibuyele kwiqondo lokushisa kunye nokusebenzisa ikhadi lokuvavanya ngokukhawuleza.
2. Beka ikhadi lovavanyo kwitafile ecocekileyo, ibekwe ngokuthe tye.
NgeSerum okanye isampuli yePlasma:
Bamba i-dropper ngokuthe nkqo kwaye udlulise i-3 yehla ye-serum okanye i-plasma (malunga ne-80 L, i-Pipette ingasetyenziswa kwimeko engxamisekileyo) kwi-specimen kakuhle , kwaye uqale ixesha.Jonga umfanekiso ongezantsi.
Ngomzekelo weGazi eliPheleleyo:
Bamba idropha ngokuthe nkqo kwaye udlulisele amathontsi e-3 egazi elipheleleyo (malunga ne-80 L) kumzekelo kakuhle, uze udibanise i-1 yethontsi yeSampuli diluent (malunga ne-40 L), kwaye uqalise isibali-xesha.Jonga umfanekiso ongezantsi.
Inyathelo lesi-3: Ukufunda
Kwimizuzu eyi-10 ~ 30, fumana isiphumo semi-quantitative ngokwekhadi eliqhelekileyo lemibala ngamehlo.
Ukutolika iziphumo
Iyasebenza: Enye purplish red streak ibonakala kumgca wolawulo (C).Ngokubhekiselele kwiziphumo ezisebenzayo, unokufumana i-semi-quantitative ngamehlo ngekhadi eliqhelekileyo lombala:
Ubunzulu boMbala vs Ugxininiso lweReferensi
| Ubunzulu bombala | Ugxininiso lweReferensi (ng / ml) |
| - | ＜0.5 |
| + - | 0.5~1 |
| + | 1~5 |
| + + | 5–15 |
| + + + | 15-30 |
| ++++ | 30~50 |
| ++++ | ＞50 |
Ayisebenzi: Akukho mkhondo obomvu wepurplish uvelayo kumgca wolawulo(C)Kule meko nceda ufunde incwadana ngononophelo kwakhona, kwaye uzame kwakhona ngekhasethi entsha yovavanyo.Ukuba imeko efanayo yenzeke kwakhona, kufuneka uyeke ukusebenzisa le bhetshi yeemveliso ngokukhawuleza kwaye uqhagamshelane nomthengisi wakho.
Ulwazi lweodolo
| Igama lemveliso | Ikati.Hayi | Ubungakanani | Umzekelo | Beka ubomi kwishelufa | Trans.& Sto.Temp. |
| I-Cardiac Troponin I Kit yoVavanyo oluKhawulezayo (Lateral Chromatography) | B032C-01 | 1 uvavanyo/ikhithi | S/P/WB | Iinyanga ezingama-24 | 2-30℃ |
| B032C-25 | Iimvavanyo ezingama-25/ikhithi |