Prokaryotic Protein Expression
Inkqubo yokubonakalisa i-prokaryotic E. coli ivunywa ngokubanzi njengeyona ndlela ineendleko eziphezulu, ekhulile ngokobuchwepheshe, kwaye isetyenziswa ngokuqhelekileyo inkqubo yokubonakalisa iprotheni.Kwi-Bioantibody, sizibophelele ekuboneleleni abathengi bethu ngeenkonzo ezibanzi, eziphuma kwindawo enye ukusuka kuhlobo lwemfuza ukuya ekubonakalisweni kweprotheyini kunye nokucocwa.Iinkonzo zethu zibandakanya ukwenziwa kwasimahla kwekhowudi nokusetyenziswa kobuxhakaxhaka bethu bobunini ukujongana nayo nayiphi na imiba enxulumene nokuchazwa okuphantsi kunye nokunganyibiliki okunokuthi kuvele ngexesha lonke lokubonakaliswa kunye nenkqubo yokucoca.Abathengi bethu badinga kuphela ukubonelela ngemfuza okanye ulandelelwano lwe-amino acid yeprotheni kwaye sinokuhambisa iprotheni ekumgangatho ophezulu nge-FAST njengeeveki ezintathu.Ukongeza, i-Bioantibody ibonelela ngokususa i-endotoxin kunye neenkonzo zokuphawula ezilungiselelwe iimfuno ezithile zabathengi bethu.Sizimisele ukuhambisa iziphumo kwaye sithembisa ukuba asiyi kuhlawulisa nayiphi na imali ukuba iprotheni yokugqibela ayibonakali.
| Izinto zeNkonzo | Umxholo woMfuniselo | Ixesha lokukhokela (BD) |
| Gene Synthesis | Ukulungiswa kweCodon, i-gene synthesis kunye ne-subcloning. | 5-10 |
| Ukuchongwa kweNgcaciso kunye noHlalutyo lokunyibilika | 1. Utshintsho kunye ne-incubation, ukubonwa kwenkcazo nge-SDS-PAGE.2. Uhlalutyo lwe-Solubility, i-SDS-PAGE kunye nokufumanisa i-WB | 10 |
| I-incubation enkulu kunye nokucoca, iprotheni yokugqibela (ubunyulu> 85%, 90%, 95%) kunye nengxelo yovavanyo oluqhelekileyo | Ukucocwa kobudlelwane (Ni ikholamu, MBP, GST) |
Ukuba i-gene idityanisiweI-bioantibody, i-plasmid eyakhiweyo iya kubandakanywa kwizinto ezinokuhanjiswa.