Inambuzane Cell Protein Expression
Inkqubo yokubonakalisa iiseli zezinambuzane yindlela eqhelekileyo esetyenziswayo yokubonakalisa i-eukaryotic yokubonakalisa iiproteni ezinkulu zemolekyuli.Xa kuthelekiswa neeseli ezanyisayo, iimeko zokukhuliswa kweeseli zezinambuzane zilula kwaye azifuni i-CO2.I-Baculovirus luhlobo lwentsholongwane ye-DNA ephindwe kabini kunye neeseli zezinambuzane njengomkhosi wendalo.Ineentlobo ezikhethekileyo zezilwanyana, ayizosuleli izilwanyana ezinomqolo, kwaye ayinabungozi ebantwini nakwimfuyo.I-sf9, eyona iqhele ukusetyenziswa njengeseli yokusingatha, ibonakala kwiplanktonic okanye ibambelele kwinkcubeko.I-sf9 ifaneleke kakhulu kwintetho yomgangatho omkhulu, kwaye ingasetyenziselwa ukusetyenzwa okulandelayo kunye nokuguqulwa kweeprotheni ezifana ne-phosphorylation, i-glycosylation, kunye ne-acylation.Inkqubo yokubonakalisa iiseli zezinambuzane inokusetyenziselwa ukubonakaliswa kofuzo oluninzi, kwaye inokuphinda iveze iiproteni eziyityhefu ezifana ne-antimicrobial peptides.
| Izinto zeNkonzo | Ixesha lokukhokela (BD) |
| Ukulungiswa kweCodon, i-gene synthesis kunye ne-subcloning | 5-10 |
| Intsholongwane yentsholongwane ye-P1 kunye nokubonakaliswa kwesikali esincinci | 10-15 |
| Intsholongwane ye-P2 yokuveliswa kwentsholongwane, ukubonakaliswa okukhulu kunye nokuhlanjululwa, ukuhanjiswa kweprotheyini ecocekileyo kunye nengxelo yovavanyo |