I-SARS-CoV-2 Ikhithi yovavanyo lwe-antibody engathathi hlangothi (iLateral chromatography)

Inkcazelo emfutshane:

Umzekelo	S/P/WB	Ifomathi	Ikhasethi
Uvakalelo	97.73%	Ukuchaza ngokuthe ngqo	98.71%
Trans.& Sto.Temp.	2-30℃ / 36-86℉	Ixesha lovavanyo	10 imiz
Inkcazo	1 Uvavanyo / Ikhithi;Iimvavanyo ezingama-25/Ikhithi

Thumela i-imeyile kuthi

Iinkcukacha zeMveliso

Iithegi zeMveliso

Ukusetyenziswa okucetywayo

I-SARS-CoV-2 Ikhithi yoVavanyo lwe-antibody engathathi hlangothi (iLateral chromatography) ilungele ukufunyaniswa ngokomgangatho we-vitro ngokukhawuleza kwe-SARS-CoV-2 engathathi hlangothi kwii-antibodies kwi-uman serum, iplasma, okanye iisampulu zegazi elipheleleyo (i-capillary okanye i-venous).Ikhithi yenzelwe njengoncedo lokuvavanya impendulo yokuzikhusela kwi-SARS-CoV-2.Ukusetyenziswa kwe-in vitro diagnostic kuphela.Ukusetyenziswa kobuchwephesha kuphela.

Umgaqo woVavanyo

I-SARS-CoV-2 Ikhithi yoVavanyo lwe-antibody engathathi hlangothi (iLateral chromatography) luvavanyo olusekwe ngokomgangatho kwi-membrane ukuze kufunyanwe izilwa-buhlungu ze-SARS-CoV-2 RBD kwi-serum, iplasma kunye negazi elipheleleyo.Isampulu yehla kwisampulu kakuhle kwaye isampulu dilution buffer yongezwa emva koko.Ii-antibodies ze-SARS-CoV-2 RBD kwisampulu zidibanisa kunye neprotein ye-RBD ebhalwe ngamasuntswana kwaye yenze i-immune complexes.Njengoko i-complex ifudukela kwi-membrane ye-nitrocellulose ngesenzo se-capillary, i-antibodies ye-RBD inokubanjwa ngenye iprotheni ye-RBD ehlanganiswe kwindawo yokuvavanya (umgca we-T), yenze umgca wesignali.Indawo yokulawula umgangatho ifakwe ibhokhwe ye-anti-chicken IgY, kunye ne-particlelabeled chicken IgY ibanjwe ukwenza i-complex kunye ne-aggregate kumgca we-C.Ukuba umgca we-C awubonakali, ubonisa ukuba umphumo awusebenzi, kwaye ukuvavanya kwakhona kuyadingeka.

Imixholo ephambili

Amacandelo anikiweyo adweliswe kwitheyibhile.

Icandelo/REF	B006C-01	B006C-25
Ikhasethi yoVavanyo	1 uvavanyo	Iimvavanyo ezingama-25
Ipadi yotywala	Iqhekeza eli-1	25 iipcs
I-Diluent yesampuli	1 ibhotile	Iibhotile ezingama-25
Imiyalelo yokusetyenziswa	Iqhekeza eli-1	Iqhekeza eli-1
ILancet elahlayo	Iqhekeza eli-1	25 PCS
Idropha	Iqhekeza eli-1	25 PCS
Isatifikethi sokuThobela	Iqhekeza eli-1	Iqhekeza eli-1

Operation Flow

Inyathelo 1: Iisampulu
Qokelela iSerum yomntu/yePlasma/Igazi elipheleleyo ngokufanelekileyo.

Inyathelo lesi-2: Uvavanyo

1. Vula ibhegi yefoyile ye-aluminium yekhadi lokuhlola.Susa ikhadi lovavanyo kwaye ulibeke ngokuthe tye etafileni.

2. Sebenzisa ipayipi elahlwayo, tshintshela i-10µL serum / okanye 10µL iplasma/ okanye 20µL igazi elipheleleyo kwisampulu yequla elikwikhasethi yovavanyo.

3. Vula ityhubhu yesithinteli ngokujija ukusuka phezulu.Bamba ibhotile yesithinteli ngokuthe nkqo kunye ne-1 cm ngaphezu kwesithinteli kakuhle.Yongeza amathontsi amathathu (malunga ne-100 µL) yesithinteli kwi-buffer kakuhle kwikhasethi yovavanyo.

Inyathelo lesi-3: Ukufunda
Imizuzu eyi-10 kamva, funda iziphumo ngokubonakalayo.(Qaphela: yenzaHAYIfunda iziphumo emva kwemizuzu eyi-15!)

Ividiyo

Isiphumo sokutolika

Isiphumo Esihle

Ukuba zombini umgca we-C wolawulo lomgangatho kunye nomgca we-T wokufunyanwa uyavela, oko kuthetha ukuba i-SARS-CoV-2 i-anti-neutralizing antibodies ichongiwe, kwaye isiphumo silungile sokunciphisa izilwa-buhlungu.

Isiphumo esibi

Ukuba kuphela umgca we-C wokulawula umgangatho uvela kwaye umgca we-T wokubhaqa awubonisi mbala, oko kuthetha ukuba i-SARS-CoV-2 i-neutralizing antibodies ayifunyaniswanga kwaye isiphumo sibi.

Isiphumo esingasebenziyo

Ukuba ukulawulwa komgangatho we-C umgca awukwazi ukubonwa, umphumo awuvumelekile kungakhathaliseki ukuba kukho umboniso wokukhangela umgca, kwaye uvavanyo kufuneka luphindwe.

Ulwazi lweodolo

Igama lemveliso	Ikati.Hayi	Ubungakanani	Umzekelo	Beka ubomi kwishelufa	Trans.& Sto.Temp.
I-SARS-CoV-2 Ikhithi yovavanyo lwe-antibody engathathi hlangothi (iLateral chromatography)	B006C-01	Uvavanyo olu-1/ikhithi	S/P/WB	Iinyanga ezili-18	2-30℃ / 36-86℉
	B006C-25	Iimvavanyo ezingama-25/ikhithi	S/P/WB	Iinyanga ezili-18	2-30℃ / 36-86℉