Uvavanyo lwe-antigen ye-One Step Mpxv yoBugcisa obuQhelekileyo kunye ne-CE
Nokuba ngumthengi omtsha okanye umthengi omdala, Sikholelwa kubudlelwane obude kakhulu kunye nobudlelwane obuthembekileyo kwiNtengiso yoBugcisa boMzimba weFactory.I-MpxvUvavanyo lwe-antigen nge-CE, Thina, ngenzondelelo enkulu nokuthembeka, sizimisele ukukunika iinkonzo ezigqibeleleyo kunye nokuqhubela phambili nawe ukudala ikamva eliqaqambileyo.
Nokuba ngumthengi omtsha okanye umthengi omdala, Sikholelwa kubudlelwane obude kakhulu nobothembekileyoI-Monkeypox, I-Mpxv, Zihlala ixesha elide kwaye zikhuthaza kwihlabathi liphela. Naphantsi kwaziphi na iimeko, imisebenzi ephambili ayinyamalali ngexesha elifutshane, yinto efanelekileyo kuwe, ngokomgangatho omangalisayo. Ikhokelwa ngumgaqo woBuchule, ukusebenza kakuhle, umanyano kunye notshintsho. Ishishini lenza imizamo emangalisayo yokwandisa urhwebo lwalo lwamazwe ngamazwe, ukuphakamisa ishishini lalo, ukuphucula nokuphucula ubungakanani balo bokuthumela ngaphandle. Siqinisekile ukuba siya kuba nethuba elihle kwaye lisasazwe kwihlabathi liphela kwiminyaka ezayo.
Iinkcukacha zemveliso
Ukusetyenziswa Okucetywayo:
II-MonkeypoxIkhithi yoVavanyo oluKhawulezayo lwe-Antigen yeVirus isetyenziselwa ukufumanisa umgangatho we-I-Monkeypoxi-antigen kwi-serum yomntu, i-lesion exudate okanye iisampulu ze-scab. Yenzelwe ukusetyenziswa ekuxilongweni kwe-in vitro kuphela.
Imigaqo yoVavanyo:
Xa isampuli icutshungulwa kwaye yongezwa kwiqula lesampuli, ii-antigen zentsholongwane ye-monkeypox kwisampuli ziyasebenzisana ne-conjugate ye-antibody-labeled virus eyenza i-antigen-antibody color particle.
Iicomplexes. Ezi complexes zihamba kwi-membrane ye-nitrocellulose ngesenzo se-capillary ukuya kumgca wovavanyo, apho zibanjwa khona zii-antibodies ze-monoclonal anti-monkeypox virus zegundane. Umgca wovavanyo onemibala ubonakala kwifestile yesiphumo ukuba ii-antigens ze-monkeypox virus zikhona kwisampuli kwaye ubunzulu buxhomekeke kubungakanani be-antigen ye-monkeypox virus. Xa ii-antigens ze-monkeypox virus kwisampuli zingekho okanye zingaphantsi komda wokufunyanwa, akukho band inombala ebonakalayo kumgca wovavanyo wesixhobo. Oku kubonisa isiphumo esibi. Umgca wovavanyo okanye umgca wolawulo awubonakali kwifestile yesiphumo ngaphambi kokusebenzisa isampuli. Umgca wolawulo obonakalayo uyafuneka ukubonisa ukuba isiphumo siyasebenza.
Isiqulatho esiPhambili
Izinto ezibonelelweyo zidweliswe kwitheyibhile.
| I-REFREF yeCandelo | B031C-01-01 | B031C-01-25 | B031C-01-25 |
| Ikhasethi yovavanyo | Uvavanyo olu-1 | Uvavanyo lwe-5 | Uvavanyo lwe-25 |
| Isitshisi esilahlwayo | Isiqwenga esi-1 | Iziqwenga ezi-5 | Iziqwenga ezingama-25 |
| Isisombululo sokukhupha isampulu | Ibhotile e-1 | Ibhotile ezi-5 | Ibhotile engama-25 |
| Iswabhu yokulahla | Isiqwenga esi-1 | Iziqwenga ezi-5 | Iziqwenga ezingama-25 |
| Imiyalelo yokusebenzisa | Isiqwenga esi-1 | Isiqwenga esi-1 | Isiqwenga esi-1 |
| Isatifikethi sokuvumelana | Isiqwenga esi-1 | Isiqwenga esi-1 | Isiqwenga esi-1 |
Ukuhamba Komsebenzi
Funda imiyalelo kakuhle ngaphambi kokuvavanya uze uzise ikhasethi kunye nesampuli kubushushu begumbi. Xa sele ukulungele ukuvavanya, vula isikhwama esikwinotshi uze ususe isixhobo. Beka isixhobo sokuvavanya kwindawo ecocekileyo, ethe tyaba.
I-Monkeypox Virus Antigen Rapid Test Kit inokwenziwa kwi-serum, i-lesion exudate okanye iisampuli ze-scab
NgokubaIisampuli zeSerum:
1. Vavanya iisampulu ze-serum ngokukhawuleza emva kokuba uziqokelele. Gcina iisampulu ze-serum kwi-2°C-8°C ukuba azivavanywanga ngoko nangoko. 2°C -8°C: ukuya kuthi ga kwiintsuku ezi-5, -20°C ukuze zigcinwe ixesha elide.
2. Ngaphambi kokuvavanya, zisa iisampulu ezikhenkcezisiweyo kubushushu begumbi kancinci uze uzixube kancinci. Iisampulu eziqulethe izinto ezibonakalayo kufuneka zicace ngokuzicoca ngaphambi kokuvavanya.
3. Zalisa i-dropper yeplastiki ngesampuli. Ubambe i-dropper ngokuthe nkqo, khupha i-50-60µL ye-serum kwityhubhu yesisombululo sokukhupha isampuli.
4. Xuba isampuli kunye nesicombululo ngokujika kancinci ityhubhu ijonge ezantsi, yiyeke ime umzuzu. Cinezela ityhubhu ukuze wongeze amathontsi ama-3 (malunga ne-100 μL) emthonjeni wesampuli yekhasethi yovavanyo.
5. Funda iziphumo ngokujonga emva kwemizuzu eli-15-20. Iziphumo azisebenzi emva kwemizuzu engama-20.
Qaphela: Ziphephe ii-freeze-thaw cycles ezininzi kwaye ungasebenzisi iisampulu ezibonisa i-gross lipoemia, i-gross hemolysis okanye i-turbidity ukuze uphephe ukuphazamiseka ekutolikweni kweziphumo.
NgokubaIisampulu ze-lesion exudate okanye i-scab:
1. Sula iisampulu ze-exudate yesilonda okanye iisampulu ze-scab nge-swab.
2. Faka iswabhu kwityhubhu yesisombululo sokukhupha isampuli uze uyifake phezulu nasezantsi kulwelo ubuncinane imizuzwana eli-15, uze ubambe iswabhu ezantsi kwityhubhu uze uyijikelezise izihlandlo ezi-5.
3. Susa i-swab ngelixa ucinezela amacala etyhubhu ukuze ukhuphe ulwelo kwi-swab. Cinezela isiciko somlomo ngokuqinileyo kwityhubhu yesisombululo equlethe isampuli ecutshungulweyo. Xuba kakuhle ngokujikelezisa okanye ngokushukumisa ezantsi kwetyhubhu.
4. Xuba isampuli ngokuyijika kancinci ityhubhu ijonge ezantsi, ucinezele ityhubhu ukuze wongeze amathontsi ama-3 (malunga ne-100 μL) kwiqula lesampuli yekhasethi yovavanyo, uze uqale ukubala.
5. Funda iziphumo ngokujonga emva kwemizuzu eli-15-20. Iziphumo azisebenzi emva kwemizuzu engama-20.
Ukutolika iziphumo
Ulwazi lwe-oda
| Igama lemveliso | Ikati. Hayi | Ubungakanani | Isampuli | Beka ubomi kwishelufa | Ubushushu beTrans. kunye neSto. |
| Ikhithi yoVavanyo oluKhawulezayo lweNtsholongwane yeMonkeypox Virus | B031C-01 | Uvavanyo/ikhithi e-1 | Iisampulu ze-serum, i-lesion exudate okanye i-scab | Iinyanga ezingama-24 | 2-30℃ |
| B031C-05 | Uvavanyo/ikhithi ezi-5 | ||||
| B031C-25 | Uvavanyo/ikhithi engama-25 |
Nokuba umthengi omtsha okanye umthengi omdala, Sikholelwa kubudlelwane obude kakhulu kunye nobudlelwane obuthembekileyo kwiNgcali yeFactory yaseTshayina ethengiswa ngexabiso eliphantsi Inyathelo elinyeI-MpxvUvavanyo lwe-Igg/Igm kunye ne-CE, Thina, ngenzondelelo enkulu nokuthembeka, sizimisele ukukunika iinkonzo ezigqibeleleyo kunye nokuqhubela phambili nawe ukudala ikamva eliqaqambileyo.
I-China yobungcali, i-China Mpxv, i-Monkey, Zihlala ziqinile kwaye zikhuthaza kwihlabathi liphela. Akukho nanye imeko enyamalala imisebenzi ephambili ngexesha elifutshane, yinto efanelekileyo kuwe ngokwakho enomgangatho omangalisayo. Ikhokelwa ngumgaqo woBuchule, ukusebenza kakuhle, umanyano kunye notshintsho. ishishini lenza imizamo emangalisayo yokwandisa urhwebo lwalo lwamazwe ngamazwe, ukuphakamisa ishishini lalo, ukuphucula nokuphucula ubungakanani balo bokuthumela kwamanye amazwe. Siqinisekile ukuba siya kuba nethuba elihle kwaye lisasazwe kwihlabathi liphela kwiminyaka ezayo.
